Molecular Biology - Nucleic Acids Extraction and purification of nucleic acids on columns Extraction and purification of DNA and RNA Photo Activation Universal Light System - PAUL

Photo Activation Universal Light System - PAUL

An open platform for different sample containers. A laboratory system for photo labeling the DNA/RNA of dead organisms through the use of photo active intercalating reagents. The Photo Activation Universal Light system combines high power LED with the proper optical alignment of the experimental support to ensure the maximum efficiency in the binding of the reagent to DNA.

• Compatible with microplates, Petri dishes, membrane filters, culture flasks and boxes, etc
• Timer function and light intensity are programmable using the device's keypad
• Standard base included, a thinner base is available to maximize interior space and samples exposition (see below)
• Thermally stable and inert base material: maintains temperature during incubation and protects your samples
• Robust and compact device

Variants and accessories

PAUL system

Reaction Buffers for viability PCR

• Séparation et purification rapides d'ADN génomique et d'ARN totaux à partir d'échantillons environnementaux, de sols et de selles
• L'échantillon environnemental est ajouté directement dans un tube contenant des billes et une solution de lyse
• Effet combiné de deux procédés, un broyage mécanique par billes et une lyse enzymatique
• Après purification, les acides nucléiques sont directement utilisables pour la digestion enzymatique, l'hybridation, la rétrotransciption et l'amplification PCR
• Nécessite un agitateur vortex ou un broyeur à bille (non fournis)

For optimum sample treatment during reagent incubation.
One of the critical steps in all vPCR workflows is sample and reagent incubation in the dark. During this treatment it is vital to maximize reagent diffusion trough dead cells, spores, cysts and microbial aggregates. On the other hand, this procedure is also necessary to prevent viable cell damage. For this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance need to be under control. See below three different reaction buffers: Standard, Standard Plus and Anaerobic, optimizing this procedure for different applications.

Specific terms
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