Molecular Biology - Nucleic Acids Reagents for RNA Reverse transcriptases

Reverse transcriptases

cDNA Synthesis: Reverse Transcriptases

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• Two reverse transcriptases for various applications
• RNase inhibitors

Kits and mix for FIREScript RT retrotranscription

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• Stable for 30 days at room temperature
• The reaction preparation is made at room temperature
• Resists up to 50 °C for high efficiency and a better sensibility
• Reproducible
• Reaction time: 15 to 30 minutes

RIBOGRIP RNase Inhibitor

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Available in enzyme version (20,000 units at 200 U/µl) with reaction buffer and dNTPs, in kit version with reaction buffer, oligo-dT, dNTPs, random hexamers and RNase inhibitor and in ready-to-use Mix version (only for FastGene scriptase II).

smART Reverse transcriptase

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• Reduced RNase H activity
• Use from 37 °C to 65 °C
• smART Enzyme (10 000 units at 200U/µl) with reaction buffer and DTT
• Applications: RT-PCR et RT-qPCR
• Enzyme optimized for cDNA fragment synthesis up to 7 kb