Extraction and purification genomic DNA -

NucleoSpin MACHEREY-NAGEL kit for DNA extraction from various samples

• DNA extraction from various samples (eukaryotic cells, bacteria, yeast, stool, urine, biopsies, bloodstains, hair, mouth swabs)
• Extraction on silica membrane column by centrifugation
• For fragment sizes from 200 bp to 50 kbp
• Yield of 20 to 35 µg

NucleoSpin DNA extraction kit for tissues and organs MACHEREY-NAGEL

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• Extraction of DNA from tissues or organs
• Extraction on silica membrane column by centrifugation
• For fragment sizes from 200 bp to 50 kbp
• Output from 1 to 30 µl

NucleoSpin plant DNA extraction kit MACHEREY-NAGEL

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• DNA extraction from plant samples
• Extraction on silica membrane column by centrifugation
• Sample quantity:
- < 100 mg wet weight and < 20 mg dry weight for the Mini kit
- < 400 mg wet weight and < 80 mg dry weight for the Midi kit
- < 1500 mg wet weight and < 300 mg dry weight for the Maxi kit
• For fragment sizes from 50 bp to 50 kpb
• Performance:
- 1 to 30 µg for the Mini kit
- 10 to 100 µg for Midi kit
- 50 to 300 µg for Maxi size kit

NucleoSpin blood DNA extraction kit MACHEREY-NAGEL

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• DNA extraction from blood, plasma or serum
• Extraction on silica membrane column by centrifugation
• Sample quantity:
- 5 to 200 µl for Mini kit
- 0.2 to 2 ml for Midi kit
- 2 to 10 ml for Maxi size kit
• For fragment sizes from 50 bp to 50 kpb
• Performance:
- 4 to 6 µg for Mini kit
- 40 to 60 µg for Midi kit
- 200 to 300 µg for Maxi kit

NucleoSpin food DNA extraction kits MACHEREY-NAGEL

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• DNA extraction from various samples (processed foods, soy (milk and flour), chocolate, cereals, meat)
• Extraction on silica membrane column by centrifugation
• For sample quantity < 200 mg
• For fragment sizes from 300 bp to 50 kpb
• Yield from 0.1 to 10 µg

NucleoSpin MACHEREY-NAGEL water extraction kit for environmental DNA

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• Environmental DNA extraction kit from water
• Extraction on column with silica matrix by centrifugation
• For sample quantity < 40 ml
• For fragment sizes from 100 bp to 50 kbp

Mini illustra™ bacteria genomicPrep Mini Spin kit

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• Illustra bacteria genomicPrep Mini Spin Kit
• For the rapid extraction and purification of genomic DNA from gram-positive and gram-negative bacteria
• Total DNA extraction time under 1 h
• Can be used to purify 4 to 8 µg of DNA for Gram + (from 2 x 10⁹bacteria cells)
• Kit designed for input amounts ranging from 1 × 10⁹to 4 × 10⁹bacteria cells
• A260/A280 between 1.7 and 1.9
• Contains Proteinase K

Mini illustra™ tissue and cells genomicPrep Mini Spin kit

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• Illustra tissue and cells genomicPrep Mini Spin kit
• For the rapid extraction of high molecular weight genomic DNA (gDNA) from tissues and cell cultures
• Up to 1.5 µg DNA / mg of tissue from an input tissue sample range of 5 to 50 mg
• Up to 40 µg genomic DNA for 5 millions cells (depending on cell type)
• Total DNA extraction in 90 min
• A 260/ A 280 = 1.8

Kit Nucleon™

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Mini illustra™ blood genomicPrep Mini Spin kit

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• Illustra blood genomicPrep Mini Spin Kit
• For the rapid extraction and purification of DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells
• Excellent repeatability and optimal yield (5 to 10 µg DNA from a 200 µl sample)
• Input sample volumes can vary from 50 µl to 1 ml
• DNA purity: A 260/A 280 >1.6
• Total time of preparation: 20 min (includes lysis, purification and desalting)

GeneMATRIX DNA Purification Kits

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• GeneMATRIX is a new generation resin selectively binding nucleic acids to silica composite structures
• Rapid Protocol
• For High purity genomic DNA, free of any contaminant (RNA, proteins, lipids, dyes, detergents, organic enzyme inhibitors, buffers, salts, divalent cations, etc.)...)
• DNA ready for use at the end of the procedure thanks to a low-salt elution buffer

GenUP gDNA

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• Genomic DNA extraction from eukaryotic cells
• Starting material: tissues, cells, rodent tail, buccal swaps and paraffin samples
• Fast and simple procedure
• Extraction time: approximately 8 min after lysis
• Binding capacity: 100 µg DNA
• Pure DNA, ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing

PerfectPure DNA™ kit

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• Uses columns
• Extraction performed in 30 minutes
• Max. DNA: 10 ug
• Recovery of 60 to 90% of DNA for fragments up to 50 kb
• A 260 / A280 between 1.7 and 2

GENUP plant DNA

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• DNA extraction from plant material (fresh, frozen, leaves, roots, buds and flowers)
• Pre-filtration to get rid of non-lysed material
• 3 lysis buffers available in the kit to find the optimal conditions depending on the type of tissue
• Quick and Simple
• Extraction / Purification: approx. 40 min
• Binding ability of the column: approx. 50 µg DNA
• Pure DNA ready to be used for all enzymatic reactions, sequencing

GENUP bacteria gDNA

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• Extraction of DNA from bacteria (Gram + or -)
• Extraction/Purification: approx. 45 min.
• Quick and simple
• Combined action of lysozyme and a proteolytic lysis
• Binding capacity of the column: 50 micrograms DNA
• Pure DNA ready to be used for all enzymatic reactions, sequencing