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Detection of caspase activity and induction of apoptosis

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• NucView® caspase-3 substrates for real-time detection of apoptosis in intact cells
• N'interfèrent pas avec l'activité des caspases, ce qui permet de suivre l'activité des caspases en temps réel
• Can be fixed with formaldehyde after staining, but cannot be used to stain fixed cells or tissues
• Pour les systèmes de cytométrie en flux, de microscopie à fluorescence ou d'imagerie des cellules vivantes

+ Substrat enzymatique de la caspase-3/7, le peptide DEVD, attaché à un colorant ADN fluorogène : avant le clivage, le colorant est non fluorescent et incapable de se lier à l'ADN
+ The substrate enters the cell cytoplasm where it is cleaved by caspase-3 in apoptotic cells to release the fluorogenic DNA dye, which colours the nucleus
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